Development of EMS-induced mutagenized groundnut population and discovery of point mutations in the ahFAD2 and Ara h 1 genes by TILLING

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dc.authorscopusid57364290900
dc.authorscopusid57219186084
dc.authorscopusid57211243492
dc.authorscopusid8157149200
dc.authorscopusid34868921600
dc.contributor.authorKaraman, Kürşat
dc.contributor.authorKızıl, Sibel
dc.contributor.authorBaşak, Merve
dc.contributor.authorUzun, Bülent
dc.contributor.authorYol, Engin
dc.date.accessioned2024-08-20T20:23:12Z
dc.date.available2024-08-20T20:23:12Z
dc.date.issued2021
dc.departmentAntalta Belek Üniversitesien_US
dc.description.abstractReducing allergenicity and increasing oleic content are important goals in groundnut breeding studies. Ara h 1 is a major allergen gene and Delta(12)-fatty-acid desaturase (FAD2) is responsible for converting oleic into linoleic acid. These genes have homoeologues with one copy in each subgenome, identified as Ara h 1.01, Ara h 1.02, ahFAD2A and ahFAD2B in tetraploid groundnut. To alter functional properties of these genes we have generated an Ethyl Methane Sulfonate (EMS) induced mutant population to be used in Targeting Induced Local Lesions in Genomes (TILLING) approach. Seeds were exposed to two EMS concentrations and the germination rates were calculated as 90.1% (1353 plants) for 0.4% and 60.4% (906 plants) for 1.2% EMS concentrations in the M1 generation. Among the 1541 M2 mutants, 768 were analyzed by TILLING using four homoeologous genes. Two heterozygous mutations were identified in the ahFAD2B and ahFAD2A gene regions from 1.2% and 0.4% EMS-treated populations, respectively. The mutation in ahFAD2B resulted in an amino acid change, which was serine to threonine predicted to be tolerated according to SIFT analysis. The other mutation causing amino acid change, glycine to aspartic acid was predicted to affect protein function in ahFAD2A. No mutations were detected in Ara h 1.01 and Ara h 1.02 for both EMS-treatments after sequencing. We estimated the overall mutation rate to be 1 mutation every 2139 kb. The mutation frequencies were also 1/317 kb for ahFAD2A in 0.4% EMS and 1/466 kb for ahFAD2B in 1.2% EMS treatments. The results demonstrated that TILLING is a powerful tool to interfere with gene function in crops and the mutagenized population developed in this study can be used as an efficient reverse genetics tool for groundnut improvement and functional genomics. © 2021 by Japan Oil Chemists’ Society.en_US
dc.description.sponsorshipAkdeniz Üniversitesi, (FYL-2017-2990)en_US
dc.identifier.doi10.5650/jos.ess21075
dc.identifier.endpage1640en_US
dc.identifier.issn1345-8957
dc.identifier.issn1345-8957
dc.identifier.issue11en_US
dc.identifier.pmid34732635en_US
dc.identifier.scopus2-s2.0-85120712769en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1631en_US
dc.identifier.urihttps://doi.org/10.5650/jos.ess21075
dc.identifier.urihttps://hdl.handle.net/20.500.14591/19
dc.identifier.volume70en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherJapan Oil Chemists Societyen_US
dc.relation.ispartofJournal of Oleo Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMutationen_US
dc.subjectOleic aciden_US
dc.subjectPeanuten_US
dc.subjectReverse geneticsen_US
dc.subjectAllergensen_US
dc.subjectAntigens, Planten_US
dc.subjectArachisen_US
dc.subjectFatty Acid Desaturasesen_US
dc.subjectGenome, Planten_US
dc.subjectLinoleic Aciden_US
dc.subjectMembrane Proteinsen_US
dc.subjectOleic Aciden_US
dc.subjectPeanut Hypersensitivityen_US
dc.subjectPlant Proteinsen_US
dc.subjectPoint Mutationen_US
dc.subjectReverse Geneticsen_US
dc.subjectAmino acidsen_US
dc.subjectLinoleic aciden_US
dc.subjectOilseedsen_US
dc.subjectacyl coenzyme A desaturaseen_US
dc.subjectallergenen_US
dc.subjectAra h 1 protein, Arachis hypogaeaen_US
dc.subjectlinoleic aciden_US
dc.subjectmembrane proteinen_US
dc.subjectoleic aciden_US
dc.subjectplant antigenen_US
dc.subjectplant proteinen_US
dc.subjectAllergen genesen_US
dc.subjectAllergenicityen_US
dc.subjectAmino-acidsen_US
dc.subjectAra h 1en_US
dc.subjectDesaturaseen_US
dc.subjectEthyl methane sulphonateen_US
dc.subjectMajor allergensen_US
dc.subjectMutationen_US
dc.subjectPoint mutationsen_US
dc.subjectReverse geneticsen_US
dc.subjectArachisen_US
dc.subjectgeneticsen_US
dc.subjectimmunologyen_US
dc.subjectmetabolismen_US
dc.subjectpeanut allergyen_US
dc.subjectplant genomeen_US
dc.subjectpoint mutationen_US
dc.subjectproceduresen_US
dc.subjectreverse geneticsen_US
dc.subjectGenesen_US
dc.titleDevelopment of EMS-induced mutagenized groundnut population and discovery of point mutations in the ahFAD2 and Ara h 1 genes by TILLINGen_US
dc.typeArticleen_US

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